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:: Volume 13, Issue 1 (5-2024) ::
gebsj 2024, 13(1): 10-21 Back to browse issues page
Identification of epiphyte bacteria isolated from sunflower in West Azarbaijan province and investigation of their ability to growth promotion
Nabi Khezrinejad , Gholam Khodakaramian * , Fatemeh Shahryari
Department of Plant Protection, Faculty of Agriculture, Bu Ali Sina University, Hamadan, Iran , khodakaramian@yahoo.com
Abstract:   (576 Views)
In order to identify epiphyte bacteria and investigate their effect on growth promoting, samples were randomly collected from the leaves and stems of sunflower plants before the flowering stage from West Azarbaijan province. 250 bacterial strains were isolated from the surface of organs (epiphytes). Four tests of indole acetic acid, ammonia, siderophore production, and phosphate solubility ability were used for initial screening and selection of strains with higher ability. The results showed that among 250 strains, 100 strains were positive in at least one out of the four tests and were selected as growth enhancing strains. The identification of representative bacteria was done based on the comparison of phenotypic characteristics and sequencing of 16S rRNA and gyrB genes. The results showed that the representatives of the selected strains were Burkholderia multivorans, Paenibacillus polymyxa, Pantoea conspicua, Pseudomonas fluorescens, Escherichia coli, Pantoea agglomerans, Bacillus subtilis, Bacillus thuringiensis, Sphingomonas aquatica. S. aquatica was reported for the first time in the world and P. polymyxa, P. conspicua and B. multivorans species were reported for the first time from Iran as species associated with sunflower. The ability to stimulate growth in plants by bacteria isolated in greenhouse conditions was investigated using the representatives of the groups of strains. The results showed that the studied treatments had a significant difference at the statistical 1% probability. Among the studied strains, E71 (P. fluorescens) showed the highest effect (54%) in increasing growth factors in greenhouse conditions, and E48 and E80 strains had less effect than the control.
Keywords: Epiphyte bacteria, Growth promoting, Sunflower, West Azarbaijan
Full-Text [PDF 657 kb]   (160 Downloads)    
Type of Study: Research | Subject: Biosafety
Received: 2023/12/21 | Accepted: 2024/04/29 | Published: 2024/08/28
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Khezrinejad N, Khodakaramian G, Shahryari F. Identification of epiphyte bacteria isolated from sunflower in West Azarbaijan province and investigation of their ability to growth promotion. gebsj 2024; 13 (1) :10-21
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Volume 13, Issue 1 (5-2024) Back to browse issues page
دوفصل نامه علمی-پژوهشی مهندسی ژنتیک و ایمنی زیستی Genetic Engineering and Biosafety Journal
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