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Report on the occurrence and genetic diversity of melon yellow spot virus isolates in various provinces of Iran
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Mehrdad Salehzadeh * , Alireza Afsharifar  |
| Plant Virology Research Center, School of Agriculture, Shiraz University, Shiraz, Iran & Plant Virology Research Center, School of Agriculture, Shiraz University, Shiraz, I. R. Iran , mehrdadsalehzadeh@gmail.com |
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Abstract: (1009 Views) |
| In a survey conducted in spring and summer 2024 across greenhouses in Isfahan, Shiraz, Bandar Abbas, Bushehr, and Yazd, severe symptoms such as yellow spots, mosaic patterns, leaf deformity, yellowing, fruit necrosis, and chlorotic ring spots were noted on cultivated C. sativus plants. To assess contamination with orthotospoviruses, total genomic RNA was extracted from five independent isolates from each area, along with an asymptomatic negative control, confirmed free of orthotospoviruses through molecular analysis. RT-PCR using degenerate primer pairs targeted orthotospoviruses, resulting in the amplification of a 420 bp fragment from infected samples. Further investigations revealed no mixed contamination with significant pathogenic viruses including Tobamoviruses, Potexviruses, Begomoviruses, and Potyviruses. Phylogenetic analysis based on nucleotide sequences indicated the presence of Melon Yellow Spot Virus. This marks the first recorded occurrence of Melon Yellow Spot Virus (MYSV) in Iran. |
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| Keywords: Iran, Orthotospovirus, Genetic diversity, Melon yellow spot virus. |
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Full-Text [PDF 619 kb]
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Type of Study: Research |
Subject:
Microrganisms and Viruses Received: 2024/12/23 | Accepted: 2025/03/1 | Published: 2025/08/4
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| References |
1. Bald-Blume, N., Bergervoet, J. H., & Maiss, E. (2017). Development of a molecular assay for the general detection of tospoviruses and the distinction between tospoviral species. Archives of virology, 162, 1519-1528.
https://doi.org/10.1007/s00705-017-3256-x [ DOI:10.1007/s00705-017-3256-x.] [ PMID] [ PMCID] 2. Chen, T. C., Lu, Y. Y., Cheng, Y. H., Chang, C. A., & Yeh, S. D. (2008). Melon yellow spot virus in watermelon: a first record from Taiwan. Plant Pathology, 57(4), 765.
https://doi.org/10.1111/j.1365-3059.2007.01791.x [ DOI:10.1111/j.1365-3059.2007.01791.x.] 3. Deng, D., McGrath, P. F., Robinson, D. J., & Harrison, B. D. (1994). Detection and differentiation of whitefly-transmitted geminiviruses in plants and vector insects by the polymerase chain reaction with degenerate primers. Annals of applied Biology, 125(2), 327-336.
https://doi.org/10.1111/j.1744-7348.1994.tb04973.x [ DOI:10.1111/j.1744-7348.1994.tb04973.x.] 4. Gibbs, A., & Mackenzie, A. (1997). A primer pair for amplifying part of the genome of all potyvirids by RT-PCR. Journal of Virological Methods, 63(1-2), 9-16.
https://doi.org/10.1016/S0166-0934(96)02103-9 [ DOI:10.1016/S0166-0934(96)02103-9.] [ PMID] 5. Gholizadeh-Roshanagh, S., Nourinejhad Zarghani, S., Aminian, H., Jafari, M., & Ramshini, H. (2017). Imported infected cucurbit seeds provoked the establishment and spread of central Europe isolates of Zucchini yellow mosaic virus in Varamin (Sothern Tehran, Iran). Genetic Engineering and Biosafety Journal, 5(2), 91-99. https://dor.isc.ac/dor/20.1001.1.25885073.1395.5.2.10.7. 6. Iwaki, M., Honda, Y., Hanada, K., Tochihara, H., Yonaha, T., Hokama, K., & Yokoyama, T. (1984). Silver mottle disease of watermelon caused by tomato spotted wilt virus. Plant Disease, 68(11), 1006-1008. DOI: 10.1094/PD-68-1006. [ DOI:10.1094/PD-68-1006] 7. Jensen, S. E. (2000). Insecticide resistance in the western flower thrips, Frankliniella occidentalis. Integrated Pest Management Reviews, 5(2), 131-146.
https://doi.org/10.1023/A:1009600426262 [ DOI:10.1023/A:1009600426262.] 8. Kato, K., Handa, K., & Kameya-Iwaki, M. (2000). Melon yellow spot virus: a distinct species of the genus Tospovirus isolated from melon. Phytopathology, 90(4), 422-426.
https://doi.org/10.1094/PHYTO.2000.90.4.422 [ DOI:10.1094/PHYTO.2000.90.4.422.] [ PMID] 9. Ghosh, A., Jagdale, S. S., Basavaraj, Dietzgen, R. G., & Jain, R. K. (2020). Genetics of Thrips palmi (Thysanoptera: Thripidae). Journal of Pest Science, 93, 27-39.
https://doi.org/10.1007/s10340-019-01160-2 [ DOI:10.1007/s10340-019-01160-2.] 10. Li, Y., Tan, G., Lan, P., Zhang, A., Liu, Y., Li, R., & Li, F. (2018). Detection of tobamoviruses by RT-PCR using a novel pair of degenerate primers. Journal of Virological Methods, 259, 122-128.
https://doi.org/10.1016/j.jviromet.2018.06.012 [ DOI:10.1016/j.jviromet.2018.06.012.] [ PMID] 11. Stadejek, T., Stankevicius, A., Murtaugh, M. P., & Oleksiewicz, M. B. (2013). Molecular evolution of PRRSV in Europe: current state of play. Veterinary microbiology, 165(1-2), 21-28.
https://doi.org/10.1016/j.vetmic.2013.02.029 [ DOI:10.1016/j.vetmic.2013.02.029.] [ PMID] 12. Maris, P. C., Joosten, N. N., Goldbach, R. W., & Peters, D. (2004). Tomato spotted wilt virus infection improves host suitability for its vector Frankliniella occidentalis. Phytopathology, 94(7), 706-711.
https://doi.org/10.1094/PHYTO.2004.94.7.706 [ DOI:10.1094/PHYTO.2004.94.7.706.] [ PMID] 13. McLeish, M. J., Zamfir, A. D., Babalola, B. M., Peláez, A., Fraile, A., & García-Arenal, F. (2022). Metagenomics show high spatiotemporal virus diversity and ecological compartmentalisation: Virus infections of melon, Cucumis melo, crops, and adjacent wild communities. Virus Evolution, 8(2), 1-16.
https://doi.org/10.1093/ve/veac095 [ DOI:10.1093/ve/veac095.] [ PMID] [ PMCID] 14. Quito-Avila, D. F., Peralta, E. L., Martin, R. R., Ibarra, M. A., Alvarez, R. A., Mendoza, A., & Ochoa, J. (2014). Detection and occurrence of melon yellow spot virus in Ecuador: an emerging threat to cucurbit production in the region. European journal of plant pathology, 140, 193-197.
https://doi.org/10.1007/s10658-014-0454-1 [ DOI:10.1007/s10658-014-0454-1.] 15. Rojas, M. R., Gilbertson, R. L., Russell, D. R., & Maxwell, D. P. (1993). Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminiviruses. Plant Disease, 77(4), 340-347. http://dx.doi.org/10.1094/PD-77-0340. [ DOI:10.1094/PD-77-0340] 16. Rotenberg, D., Jacobson, A. L., Schneweis, D. J., & Whitfield, A. E. (2015). Thrips transmission of tospoviruses. Current opinion in virology, 15, 80-89.
https://doi.org/10.1016/j.coviro.2015.08.003 [ DOI:10.1016/j.coviro.2015.08.003.] [ PMID] 17. Sievers, F., & Higgins, D. G. (2018). Clustal Omega for making accurate alignments of many protein sequences. Protein Science, 27(1), 135-145.
https://doi.org/10.1002/pro.3290 [ DOI:10.1002/pro.3290.] [ PMID] [ PMCID] 18. Salehzadeh M., Afsharifar A., Dehghanpour Farashah S. and Rezaei M. 2022. The first report of the Chilli leaf curl virus and its beta satellite from bell peppers and tomatoes from the central provinces of Iran. Iranian Journal of Plant Pathology 57(4), 337-341. [ DOI:10.22034/ijpp.2022.549473.384.] 19. Salehzadeh M., Afsharifar A., dehghanpour farashah S., and rezaei,. 2021. The first report of mild pepper mild mottle virus in mixed infection with Tomato brown rugose fruit virus from pepper in Isfahan province. Iranian Journal of Plant Pathology, 57(3), 263-267. doi: 10.22034/ijpp.2022.549587.385. 20. Salehzadeh M., Afsharifar A., & dehghanpour farashah S. 2024. The First Report of Alfalfa mosaic virus Occurrence on Pepper and Tomato Crops Based on Partial Nucleotide Sequence analysis of the Virus Coat Protein Open Reading Frame from Hormozgan Province. Iranian Journal of Plant Pathology, 59(1), 64-68. doi: 10.22034/IJPP.2024.2021784.441. 21. Salehzadeh, M. (2018). Survey on presence of Cucumber mosaic virus (CMV) in single and mixed infections with potyviruses in North-West of Iran. Genetic Engineering and Biosafety Journal, 7(2), 163-173. https://dor.isc.ac/dor/20.1001.1.25885073.1397.7.2.4.5. 22. Tajik Khademi, H., Khodadadi, M., Hassanpanah, D., & Hajainfar, R. (2024). Changes in fruit yield, biochemical attributes, and leaf minerals of different cucumber (Cucumis sativus L.) cultivars under foliar application of silicon nanoparticles. Environmental Science and Pollution Research, 31(29), 42012-42022.
https://doi.org/10.1007/s11356-024-33890-x [ DOI:10.1007/s11356-024-33890-x.] [ PMID] 23. Tamura, K., Dudley, J., Nei, M., & Kumar, S. (2007). MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular biology and evolution, 24(8), 1596-1599.
https://doi.org/10.1093/molbev/msm092 [ DOI:10.1093/molbev/msm092.] [ PMID] [ PMCID] 24. Takeuchi, S., Okuda, M., Hanada, K., Kawada, Y., & Kameya, M. (2001). Spotted wilt disease of cucumber (Cucumis sativus) caused by Melon yellow spot virus. Japanese Journal of Phytopathology, 67(1), 46-51.
https://doi.org/10.3186/jjphytopath.67.46 [ DOI:10.3186/jjphytopath.67.46.] 25. Takeuchi, S., Shimomoto, Y., & Ishikawa, K. (2009). First report of Melon yellow spot virus infecting balsam pear (Momordica charantia L.) in Japan. Journal of General Plant Pathology, 75(2), 154-156.
https://doi.org/10.1007/s10327-009-0143-7 [ DOI:10.1007/s10327-009-0143-7.] 26. Tamura, K., Dudley, J., Nei, M., & Kumar, S. (2007). MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24(8), 1596-1599. [ DOI:10.1093/molbev/msm092] [ PMID] [ PMCID] 27. Wu, H., Liu, M., Li, W., Wang, M., Xiu, J., Peng, B., & Gu, Q. (2024). Development and Application of Droplet Digital PCR Assay for the Detection of Watermelon Silver Mottle Virus and Melon Yellow Spot Virus. Horticulturae, 10(3), 1-11.
https://doi.org/10.3390/horticulturae10030199 [ DOI:10.3390/horticulturae10030199.]
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Salehzadeh M, Afsharifar A. Report on the occurrence and genetic diversity of melon yellow spot virus isolates in various provinces of Iran. gebsj 2025; 13 (2) :225-234 URL: http://gebsj.ir/article-1-506-en.html
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