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:: Volume 13, Issue 2 (1-2025) ::
gebsj 2025, 13(2): 256-264 Back to browse issues page
Induction of hairy roots in multi-year-old yew (Taxus baccata) seedlings using Agrobacterium rhizogenes for the production of taxol
Mahdi Mahmoudi , Seyed Ahmad Sadat Noori *
Department of Agronomy and Plant Breeding, Abu Raihan Faculty of Agricultural Technology, University of Tehran, Pakdasht, Iran , noori@ut.ac.ir
Abstract:   (1052 Views)
Yew (Taxus baccata) is a diploid plant with a chromosome number of 2n=2x=24, belonging to the order Taxales and the family Taxaceae. Among its most important secondary metabolites is taxol, a well-known anticancer drug. The aim of this study was to investigate the induction of hairy roots in perennial yew seedlings using Agrobacterium rhizogenes to optimize the production of secondary metabolites under in vitro conditions. The experiment was conducted as a factorial design in a completely randomized format with three replications. The factors examined included explant type at two levels (root and leaf) and bacterial strain at three levels (ATCC-15834, TR107, MAFE01724). The results demonstrated that the interaction of the examined factors significantly influenced the traits of hairy root induction percentage, fresh weight, and dry weight of hairy roots after two months. The highest percentage of hairy root induction, as well as fresh and dry weights, were achieved with the combination of the MAFE01724 strain and leaf explants, which were significantly higher compared to other treatment combinations. The transgenic nature of the hairy roots was confirmed by tracing a segment of the rolB gene using PCR amplification, while no corresponding band was amplified for non-transgenic (wild-type) roots. Overall, the results of this study indicate that the induction of hairy roots in perennial yew (Taxus baccata) seedlings is influenced by the explant type and bacterial strain. These findings provide an essential prerequisite for further experiments on hairy root cultures aimed at the production of pharmaceutical metabolites.
 
Keywords: Yew, Hairy root, Agrobacterium rhizogenes, Taxol
Full-Text [PDF 550 kb]   (171 Downloads)    
Type of Study: Research | Subject: Plant
Received: 2025/02/27 | Accepted: 2025/08/3 | Published: 2025/09/14
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Mahmoudi M, Sadat Noori S A. Induction of hairy roots in multi-year-old yew (Taxus baccata) seedlings using Agrobacterium rhizogenes for the production of taxol. gebsj 2025; 13 (2) :256-264
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Volume 13, Issue 2 (1-2025) Back to browse issues page
دوفصل نامه علمی-پژوهشی مهندسی ژنتیک و ایمنی زیستی Genetic Engineering and Biosafety Journal
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