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:: Volume 14, Issue 1 (9-2025) ::
gebsj 2025, 14(1): 16-30 Back to browse issues page
Effects of Titanium Dioxide Nanoparticles on the Anatomical and Biochemical Characteristics of Aptenia cordifolia at Different Developmental Stages
Masoumeh Bohli Bohl , Hanieh Mohajjel Shoja * , Samira Behboudi , Saeideh Alizadeh-Salteh
Department of Plant, Cell and Molecular Biology, Faculty of Natural Sciences, University of Tabriz, Iran. , mohajelh@yahoo.com
Abstract:   (818 Views)
Titanium dioxide nanoparticles (TiO₂) have gained attention for their photocatalytic properties and broad applications in life sciences and agriculture. However, their impact on plant growth and development remains a topic of ongoing debate. This study, utilizing a completely randomized design, investigated the effects of foliar-applied TiO₂ at concentrations of 0, 0.03%, and 0.05% on the growth, morphology, and biochemical traits of Aptenia cordifolia during its vegetative and reproductive stages. The results indicated that TiO₂ treatments reduced growth parameters, such as stem length, root length, and leaf area, during both vegetative and reproductive stages compared to controls. However, plants treated with 0.03% TiO₂ exhibited significantly higher fresh and dry weights of roots and shoots, as well as greater stomatal density, compared to those treated with 0.05%, suggesting a potential inhibitory effect of the higher concentration. In the vegetative stage, the 0.03% treatment increased photosynthetic pigments (chlorophyll a, b, total chlorophyll, and carotenoids) and peroxidase activity, while in the reproductive stage, these increases were observed with the 0.05% treatment. Additionally, the 0.05% TiO₂ treatment led to an increase in protein content in both stages. These findings demonstrate that the effects of TiO₂ are concentration- and growth stage-dependent, with lower concentrations enhancing biochemical properties, while higher concentrations adversely affected plant growth and metabolism. Therefore, careful consideration of nanoparticle concentration and application methods is essential for optimizing their effectiveness in agricultural applications.
Keywords: Antioxidant enzymes, growth parameters, photosynthetic pigments, reproductive stage, vegetative stage
Full-Text [PDF 1040 kb]   (151 Downloads)    
Type of Study: Research | Subject: Biosafety
Received: 2025/05/6 | Accepted: 2025/08/8 | Published: 2025/09/20
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Bohli Bohl M, Mohajjel Shoja H, Behboudi S, Alizadeh-Salteh S. Effects of Titanium Dioxide Nanoparticles on the Anatomical and Biochemical Characteristics of Aptenia cordifolia at Different Developmental Stages. gebsj 2025; 14 (1) :16-30
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Volume 14, Issue 1 (9-2025) Back to browse issues page
دوفصل نامه علمی-پژوهشی مهندسی ژنتیک و ایمنی زیستی Genetic Engineering and Biosafety Journal
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