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The optimization of hairy root induction in broccoli plant
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Maryam Bigdeli *   , Ahmad Sadat Noori , Ali Izadi darbandi , Mahmood Lotfi , Moslem Bahmankar |
| University of Tehran , maryambigdeli3154@yahoo.com |
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Abstract: (49 Views) |
Broccoli (Brassica oleracea var. Italica) is a well-known, cool-weather vegetable of the family Brassicaceae. This plant provided a good source of nutritional benefits. The Broccoli culture includes many critical secondary metabolites, especially those containing glucosinolates and their breakdown product. The most important medicinal metabolite in broccoli is glucosinolate, which is the reason for its pungent smell and anti-cancer properties. Inside the plant, glucosinolate-glucoraphanin is converted into sulforaphane and 1-butene,4-isothiocyanate. Regarding the importance and application of secondary metabolites, the cultivation of Hairy roots is a suitable solution for producing more of these substances. For this purpose, in this study, the optimization of the induction of hairy roots was done by using two methods, in-planta, and tissue culture. This project was conducted as a factorial experiment based on a completely randomized block design with three replicates (as Petri dishes). Factors studied included two bacterial strains (ATCC-15834 and A4), four plant genotypes (Her, Cast, Sac, and Sent), and two explant types (Hypocotyl and Cotyledon). The results showed that the combination of the three factors studied (culture×explant×strain) on the characteristic of emergence time on the induction of hairy roots was not significant. In the tissue culture technique, the (Sac×A4) treatment was faster than the other treatments studied in terms of emergence time. The transgenic nature of the hairy roots was confirmed by tracking a part of the rolB gene using the PCR reaction, while the corresponding band was not amplified for the natural and non-transgenic roots. In general, the results of the present study showed that the induction of hairy roots in Broccoli was affected by explant and bacterial strain, and these results are an important prerequisite for hairy root culture experiments with the aim of producing secondary metabolites.
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Article number: 4 |
| Keywords: Hairy roots, secondary metabolites, Broccoli |
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Type of Study: Research |
Subject:
Plant
Received: 2025/06/6 | Accepted: 2025/10/23 | Published: 2025/10/28
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