|
Isolation, Characterization, and Evaluation of Biocontrol Properties of Plant Growth-Promoting Pseudomonas Bacteria from the Tomato Rhizosphere
|
Bahareh Khoshkholgh   , Fatemeh Dehghan Nayeri * , Hassan Rasouli |
| Department of Biotechnology, Imam Khomeini International University (IKIU), Iran , nayeri@eng.ikiu.ac.ir |
|
|
Abstract: (281 Views) |
This study aims to isolate fluorescent growth-promoting bacteria of the genus Pseudomonas from the rhizosphere of tomato (Solanum lycopersicum) fields of Qazvin and Shabestar provinces, at three different soil depths. The isolates were evaluated for their ability to produce auxin, siderophore, hydrogen cyanide, and for inorganic phosphate solubilization. The highest number of isolates was found at a depth of 15 to 25 cm. The average auxin production (40.97 µg/mL) and the maximum hydrogen cyanide production capacity (more than 10 isolates) were observed in soil samples collected from Qazvin. Bacterial isolates including Q19, Q22, Q25, and Q26 showed substantial ability to inhibit the growth of the pathogenic fungus Fusarium oxysporum. Furthermore, molecular docking results revealed that the compound phenazine-1-carboxamide, with a binding energy of -8.3 kcal/mol, is capable of interacting with the SEG 1 protein of the Fusarium fungus, a finding that could be utilized in the development of new fungicides based on bacterial secondary metabolites. Overall, the results of this research demonstrate that the tomato rhizosphere is rich in plant growth-promoting bacteria with diverse biological capabilities, suitable for the development of environmentally friendly biofertilizers.
|
Article number: 6 |
| Keywords: Tomato, rhizosphere, Fusarium oxysporum, Pseudomonas |
|
|
|
Type of Study: Research |
Subject:
Divers
Received: 2026/05/7 | Accepted: 2026/06/22 | Published: 2026/06/22
|
|
|
|
|
|
|
| Add your comments about this article |
|
|
|
