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The Optimization of Hairy Root Induction in Broccoli Plant
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Maryam Bigdeli *   , Ahmad Sadat Noori , Ali Izadi darbandi , Mahmood Lotfi , Moslem Bahmankar |
| Department of Agronomy and Plant Breeding, Agriculture Faculty, University of Tehran, Paradis of Aburaihan, Iran , maryambigdeli3154@yahoo.com |
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Abstract: (108 Views) |
Broccoli (Brassica oleracea var. Italica) is a well-known plant, suitable for cold and cool climates, belonging to the Brassicaceae family. This plant is a good source of nutrition. Broccoli cultivation involves many vital secondary metabolites, particularly glucosinolates which is responsible for its pungent odor and anti-cancer properties. Within the plant, glucosinolate-glucoraphanin is converted into sulforaphane and 1-butene, 4-isothiocyanate. Given the importance and application of secondary metabolites, the cultivation of hairy roots is a suitable strategy for enhanced production of these compounds. To optimize hairy root induction, the tissue culture method was employed. This project was conducted as a factorial experiment based on a completely randomized block design with three replicates (under Petri dish culture conditions). The studied factors included two bacterial strains (ATCC-15834, A4), four plant genotypes (Heraklion, Castle Dome, Sakura, and Sentora), and two types of explants (Hypocotyl and Cotyledon). The best treatment for hairy root induction in broccoli was the combination of genotype SENT, strain A4, and cotyledon explant, which achieved 100% induction and the highest number of roots. For producing the highest biomass (fresh and dry weight), the combination of genotype HER, strain A4, and cotyledon explant showed the best result. The transgenic nature of the hairy roots was confirmed by tracking a part of the rolB gene using PCR. In general, the results of this study indicated that the induction of transgenic roots in broccoli is influenced by the explant and bacterial strain, and these findings serve as a key prerequisite for experiments related to hairy root culture aimed at producing secondary metabolites. |
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| Keywords: Hairy roots, secondary metabolites, Broccoli |
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Type of Study: Research |
Subject:
Plant
Received: 2025/06/6 | Accepted: 2025/10/23 | Published: 2025/10/28
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